Figure 9 of Wolf, Mol Vis 2008; 14:274-285.


Figure 9. The presence of XRCC1 in the surface LEC nuclei at 72 h indicates ongoing DNA repair. Antibody for the DNA repair protein, XRCC1, was applied with a secondary fluorescent antibody and P.I. red background for nuclear DNA. The lens central zone (A) and near the lens equator (B) are from non-irradiated controls and are negative for XRCC1 expression. Panel C shows positive staining in the lens LEC nuclei, seen on the left. To their right is the space between lens and cornea, and at far right is the cornea. D: The panel shows a section from the same lens near the equator. The last two panels (C and D) are 72 h post-irradiation. Magnification 40X.