Figure 8. The DNA adduct 8-OHG is present in surface LEC nuclei in the x-ray cataract. Direct antibody for 8-OHG was applied followed by secondary fluorescent antibody staining in a Carnoy’s fixed paraffin section. The non-irradiated animal’s lens is on the left and the irradiated is on the right, both observed 72 h post-irradiation at 400X total magnification. The background stain in this and subsequent sections is propidium iodide staining for nuclear DNA.