Figure 3 of Diederen, Mol Vis 2008; 14:255-261.


Figure 3. In vitro expression of cyclic guanosine monophosphate (cGMP) in the outer plexiform layer of a detached retina. Small pieces of retina were incubated with IBMX (a non-specific PDE inhibitor) and the particulate guanylyl cyclase (pGC) stimulator atrial natriuretic peptide (ANP). The expression of cGMP (cGMP: green color) in the retina was then analyzed by immunohistochemistry and nuclei were counterstained with Hoechst 33342 (red). Following stimulation with ANP in the presence of IBMX, both the detached and healthy retina showed cGMP-immunolabeling in the outer plexiform layer. Panel A shows the labeling of the outer plexiform layer of a detached retina with Hoechst. Panel B shows the cGMP stain (green) of the outer plexiform layer probably representing photoreceptor axons and dendrites. Panel C is a double staining exposure of the nuclei and cGMP. Scale bar represents 20 μm.