Table 2 of
Gu, Mol Vis 2008;
14:20-28.Table 2 of
Gu, Mol Vis 2008;
14:20-28.
Table 2. Primers used to amplify the full-length cDNA of rat Nyx and Cacna1f
Fragments of rat cDNA of Nyx and Cacna1f were amplified to cover the full length. Primers were designed according to sequences isolated in previous part.
Gene Primer Position Sequence (5'-3')
------- ------ --------- --------------------------
Nyx TN1F 1-26 GAGAAAGAAAAATAAGCAGTCAAACC
TN1R 1032-1056 GATGCTGTTGCGATCTAGGTAAAGC
TN2F 1219-1239 GAGTGGTTGCGTGATTGGATG
TN1R 1946-1971 TTCTACTTTAATTTAGGCCTGTAGGC
Cacna1f TC1F 1-25 GTGTGCAGATGGTCCTTCTATCTCC
TC1R 1461-1480 CGGGGGTGTCTGTTATGGAA
TC2F 1230-1249 GGACCTTCGGGGCTACCTGG
TC2R 2366-2385 TTGGGGAGGGTTTCCTTCAC
TC3F 2264-2287 GTGGCAACTACATCCTACTGAACG
TC3R 3559-3579 CACGCGGTACTGATGTGGATT
TC4F 3473-3497 ATCAAAACTGTGAACTGGACAAGAA
TC4R 4249-4268 CCACAGGTAAATTCCTCGCC
TC5F 4090-4111 CTTCAGGACGGCACACAGATAA
TC5R 4777-1799 TGGATGAGAAATGTGGCATAGAA
TC6F 4695-4717 AAAGATCTGGAAGCGGATAAAGC
TC6R 5703-5723 CTGCCCCTCTTGCGGTGACTG
TC7F 5468-5488 AACGCCAGGGCAGTTGTGAGG
TC7R 5990-6014 GCAGGGAATTTATTGAGCGATAGGTA
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