Table 1 of
Gu, Mol Vis 2008;
14:20-28.Table 1 of
Gu, Mol Vis 2008;
14:20-28.
Table 1. Primers used to isolate the rattus norvegicus Nyx and Cacna1f full-length cDNA
Full length cDNA of rat Nyx and Cacna1f were isolated by 5' and 3' rapid amplification the cDNA ends. Primers were designed according to available sequences and manufacturer's protocol.
Gene Purpose Primer (5'-3') Location
-------------- ----------------- ----------------------------- ------------------
Nyx 5' RACE F: AGGAGACGCTCGGGCACGCTGAAG (nt496 to nt159)
According to 3' RACE R: GCACCCTCAATCTGGGCGGCAAC (nt825 to nt847)
(NM_001100967) For middle region F: CCACAACAACCTGTCCTTTATTAC (nt337 to nt360)
R: TCAGTCCCTCTGTGGACCCAAC (nt1480 to nt1501)
Cacna1f 5' RACE F: TGGCTTCCACTCCACAATGCTGATG (nt284 to nt308)
According to 3' RACE R: CAGTGACCTGCTGGCACAGAGAACC (nt5861 to nt5885)
(NM_053701) For middle region F: AGAGGATGTCGGAATCTGAAGTCG (nt25 to nt48)
R: GGACCTTCGGGGCTACCTGGAC (nt1226 to nt1247)
F: TGGAAGCGGATAAAGCAGAAAT (nt4698 to nt4719)
R: TCAAAACTGTGAACTGGACAAGAA (nt2470 to nt2493)
F: TGGAAGCGGATAAAGCAGAAAT (nt4698 to nt4719)
R: GGGGGTGTCTGTTATGGAACCA (nt1454 to nt1475)
F: CCTTGCGAAGCGGGTTGGTTTG (nt2592 to nt2613)
R: GTGTTGAGGAGGATGAGCAGAA (nt3616 to nt3637)
F: TGGATGAGAAATGTGGCATAGAA (nt4773 to nt4775)
R:CATCAAAGCGGGAGAGAATAGACT (nt5908 to nt5931)
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