Figure 6. Western blot analysis of the conditioned medium from the corneal epithelia cells and cell lines and human tear fluid. The primary corneal epithelial cells were shown to secrete 14-3-3 σ, ζ, and γ in to the condition medium (A). E6/E7 and SV 40 transformed corneal epithelial cells and primary conjunctival epithelial cells also secrete these three isoforms (B, C, and D, respectively). The human tear fluid from normal human volunteers shows the presence of 14-3-3 η and γ isoforms but sigma is absent (H, I, and J). E, F, and G are derived form a Ponceau Red stained blot to show protein loading. The molecular weight ladder is seen in L.