Figure 3. Comparison of the expression of 14-3-3 isoforms in the primary corneal and conjunctival cells with that in cell lines as determined by western blot analyses of whole cell lysates. All the isoforms are expressed in the primary conjunctial epithelial cells but the 14-3-3 ζ is expressed weakly (A). Primary corneal stromal fibroblasts express all the 14-3-3 isoforms but the expression of η isoform is low, and σ isoform is not expressed (B). The primary corneal endothelial cells express all 14-3-3 isoforms except σ and θ is barely detectable (C). The E6/E7 transformed corneal endothelial cells express all the 14-3-3 isoforms more robustly than the primary cells, the expression of θ isoform is more evident and there is no expression of σ isoform (D).