Figure 7. mPins protein localization in the adult mouse retina by immunohistochemistry. A-C show mPins protein localization by DAB chromogen immunochemistry. A shows the DAB-immunonegative control. D-F show confocal views of mPins protein localization by immunofluorescence; E shows the immunonegative control. mPins protein is detected in all retinal nuclear layers: the ganglion cell layer (GCL), the inner nuclear layer (INL), and the outer nuclear layer (ONL). The protein is also observed in the inner plexiform layer (IPL), with weak labeling detectable in the outer plexiform layer (OPL; B and C). mPins protein is also observed in the retinal pigment epithelium (RPE) and in the choroidal melanocytes (Ch; C). Higher magnification also shows strong immunolabeling in the inner segments of the photoreceptors (IS; C). Moreover, in the INL, mPins labeling is stronger on either side of the layer than in its center (C, asterisks). Confocal views confirm this distribution of protein and show the mPins protein to be present principally in the cellular membrane of the INL and ONL cells (D and F).