Figure 5. mPins protein localization in embryonic mouse retina by immunohistochemistry. mPins protein localization is shown in embryonic mouse retina at stages E12.5 (A), E14.5 (B), E16.5 (D), and E18.5 (E and F). mPins protein distribution is also shown in lens at E14.5 (C) and E18.5 (G). Orientation of mouse eye sections is specified below the figure. From E12.5 to E18.5, mPins protein is detected in neuroblastic layer (NbL; A, B, D, E, and F). Furthermore, mPins immunolabeling is stronger in the inner part of the NbL from E16.5 (D-F). At this stage, the mPins protein is also observed in the ganglion cell layer (GCL; F). The mPins protein is also found in the lens with a specific distribution depending on the stage (C and G). At the E14.5 stage (C), immunostaining is observed in the anterior region, whereas staining is observed only in the posterior region of the lens by E18.5 (G). Moreover, mPins protein is observed in the corneal epithelium, the presumptive cornea from E12.5 (A and E). H shows Diaminobenzidine (DAB) immunonegative control.