Figure 2. mPins mRNA localization in mouse embryo by radioactive in situ hybridization. mPins labeling with dark-field illumination (left) and the corresponding bright-field (right) are shown for stages E11.5 (A), E12.5 (B), E13.5 (C), E14.5 (D), E15.5 (E), E16.5 (F), E17.5 (G) and E18.5 (H and I). Dark-field negative controls using sense (J, left) and scramble (J, right) are also shown. mPins transcripts are detected in the neuroblastic layer (NbL) of the retina from E11.5 to E18.5 of embryonic development (A-I). The corresponding bright-field views confirm these observations (black grains). At E18.5, no significant signal is detected in the ganglion cell layer (H). At the same stages, we also observe a strong signal at the posterior face of the lens, corresponding to secondary fiber cells (I, asterisk). Negative controls using sense (J, left) and scramble (J, right) probes gave no significant signal.