Figure 6. Cell cycle distribution of corneal fibroblasts treated with TSA or NaBu. In normal human corneal fibroblasts, about 64.4%, 6.5% and 29.1% were in the G₀/G₁, G₂/M, and S phase, respectively. After treatment with 400 nM TSA or 5 mM NaBu for 3 days, the proportion of cells in the G₀/G₁ phase increased to 80.9% with TSA and to 78.5% with NaBu, the proportion of cells in the G₂/M phase increased to 10.4% with TSA and 9.4% with NaBu, and the proportion of cells in the S phase reduced to 8.7% with TSA and 12.1% with NaBu.