Figure 4. TSA and NaBu suppressed the TGFβ-mediated upregulation in the expression of collagen I and α-SMA at the protein levels. As shown by the analysis (B) of western blotting (A), TGFβ1 significantly upregulated the protein levels of α-SMA and collagen I by 1.8 and 2.2 fold, respectively, while 400 nM TSA strongly inhibited the synthesis of α-SMA and collagen I by 32% and 57%, respectively, and 5 mM NaBu suppressed the synthesis of α-SMA and collagen I by 43% and 69%, respectively.