Figure 2. Trichostatin A suppressed the myofibroblastic differentiation of mouse corneal stromal cells induced by TGFβ1. Corneal fibroblasts treated with 1 ng/ml TGFβ1 for 4–5 days exhibited enhanced staining with FITC-conjugated phalloidin (A), α-SMA (B), and collagen I (C). However, the keratocytes treated with TGFβ1 in the presence of 400 nM TSA remained small with dendritic-like morphology and weak or only minimal staining of phalloidin (D), α-SMA (E), and collagen I (F). Bar, 50 µm.