Figure 11 of Zhou, Mol Vis 2008; 14:2556-2565.


Figure 11. Analysis of partially reversible inhibitory effects of TSA on the myofibroblastic differentiation of corneal stromal cells. The samples were prepared as the corneal fibroblasts treated with 400 M TSA for 4 days (marked as the TSA lane), cells treated with 400 M TSA for 4 days and further incubation in the control medium (marked as the TSA-Control lane) or in the presence of TGFβ1 (marked as the TSA-TGFβ1 lane), cells treated with 400 M TSA for 8 days (marked as the TSA-TSA lane). The expression of collagen I and α-SMA were up-regulated after the removal of TSA of 4 days, and even rescued from the inhibition of HDAC inhibitors in the presence of TGFβ1.