Figure 11. Analysis of partially
reversible inhibitory effects of TSA on the myofibroblastic
differentiation of corneal stromal cells. The samples were prepared as
the corneal fibroblasts treated with 400 M TSA for 4 days (marked as
the TSA lane), cells treated with 400 M TSA for 4 days and further
incubation in the control medium (marked as the TSA-Control lane) or in
the presence of TGFβ1 (marked as the TSA-TGFβ1 lane), cells treated
with 400 M TSA for 8 days (marked as the TSA-TSA lane). The expression
of collagen I and α-SMA were up-regulated after the removal of TSA of 4
days, and even rescued from the inhibition of HDAC inhibitors in the
presence of TGFβ1.