Figure 1. Trichostatin A prevented morphological changes of TGFβ-mediated myofibroblastic differentiation of corneal fibroblasts. Freshly isolated mouse corneal keratocytes exhibited a dendritic-like morphology when cultured in the serum-free medium (A). Serum (10% FBS) and TGFβ (1 ng/ml) induced rapid changes of cellular morphologies from dendritic to spreading and caused cells to finally assume myofibroblastic morphology (B, C). The addition of TSA suppressed the differentiation mediated by fetal bovine serum (D) and TGFβ (E, F), and the inhibition was dose-dependent as shown in (E; 100 nM TSA) and (F; 400 nM). Human quiescent corneal keratocytes (G), TGFβ induced the differentiated myofibroblasts (H), and 400 nM TSA treated corneal fibroblasts (I) exhibited similar morphological changes. (200X)