Figure 5. Luciferase expression profile following 1 IVT injection of Ad5- or Ad35-based vectors. Luciferase activity in mouse eyes following a single IVT injection of 1×10⁹ pu of either Ad5.L or Ad35.L (HI-RGD). Naive animals served as negative controls. On Days 1, 28, 60, and 120 post vector injection, eyes were harvested and luciferase activity assessed. Data are expressed as the mean±SEM (error bars) with an n=15 mice/treatment group/time point, except for Day 120, which has an n=5 mice/treatment group. The asterisk indicates a statistically significant difference between the Ad5 and Ad35 groups on Day 120 post injection (p<0.05, two-tailed Student’s t-test).