Figure 4. Effect of EGCG on intracellular H₂O₂ production of ARPE19 cells after UVA radiation exposure. ARPE19 cells pretreated with PBS (A,B) or EGCG for 2 h (C-E) were loaded with DHR 123. After being exposed to 20 J/cm² UVA radiation (B-E), cells were collected and subjected to flow cytometric analysis. Results are presented as histograms of the number of cells versus fluorescence intensity. Each histogram represents three independent experiments. F: Quantitative analyses of intracellular H₂O₂ in ARPE19 cells (n=3). The asterisk indicates p<0.05 versus UVA-exposed cells without EGCG pretreatment.