Table 2 of Mafia, Mol Vis 2008; 14:234-248.

Table 2. Molecular weights (in Dalton) of unexposed and UV-A-exposed WT αB and its three deamidated mutant proteins.

Protein Type Unexposed UV-A exposed
WT αB HMW 1.84×106 4.61×107

Crystallin 4.28×105 5.00×105




Asn78Asp HMW None 1.60×106

Crystallin 5.30×105 5.30×103




Asn146Asp HMW None 2.6×107

Crystallin 4.20×105 4.96×105




Asn78/146Asp HMW 6.60×105 3.70×107

Crystallin 5.01×105 6.23×105

Oligomer size determination of UV-A-unexposed and UV-A-exposed WT αB-crystallin and its three deamidated proteins by a dynamic light scattering method. A multiangle-light scattering instrument coupled to a HPLC system was used to determine the absolute molecular molar mass. Results used 18 different angles, and the angles were normalized with 90o detector.

Mafia, Mol Vis 2008; 14:234-248. http://www.molvis.org/molvis/v14/a29

©2008 Molecular Vision http://www.molvis.org/molvis/

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