Figure 5. Determination of secondary structure by Far-UV circular dichroism spectroscopy of UV-A-exposed and UV-A-unexposed WT- and deamidated αB-crystallin species. Far-UV CD spectra of UV-A-unexposed (^____) and UV-exposed (----) WT αB-crystallin and its three deamidated mutants. Far-UV CD spectra were determined as described in Methods. (A) WT αB-crystallin, (B) αB-Asn78Asp mutant (C) αB-Asn146Asp mutant, and (D) αB-Asn78/146Asp mutant.