Figure 4. Intrinsic Trp fluorescence determination of UV-A-exposed and UV-A-unexposed WT- and deamidated αB-crystallin species. Intrinsic Trp fluorescence spectra of UV-A-unexposed (^____) and UV-exposed (-----) WT αB-crystallin and its three deamidated mutants. Fluorescence spectra were determined as described in Methods. (A) WT αB-crystallin, (B) αB-Asn78Asp mutant, (C) αB-Asn146Asp mutant, and (D) αB-Asn78/146Asp mutant.