Figure 3. ANS-binding and fluorescence determination of UV-A-exposed and UV-A-unexposed WT- and deamidated αB-crystallin species. Fluorescence spectra of UV-A-unexposed (^____) and UV-A-exposed (-------; at 50 J/cm²) of WT αB-crystallin and its three deamidated mutants after ANS binding. Fluorescence spectra following ANS-binding were determined as described in Methods. (A) WT αB-crystallin, (B) αB-Asn78Asp mutant, (C) αB-Asn146Asp mutant, and (D) αB-Asn78/146Asp mutant.