Figure 1. SDS–PAGE analysis of UV-A-exposed and unexposed WT αB-crystallin and its three deamidated mutant species. After UV-A-exposure of varying doses (0, 20, and 50 J/cm², shown at the bottom of the gel), the WT αB-crystallin and its three deamidated mutant proteins (αB-Asn78Asp, αB-Asn146Asp, and αB-Asn78/146Asp) were analyzed. Increased dimerization of each protein and degradation, particularly in the deamidated species, were observed following UV-A-exposure.