Figure 5 of Chen, Mol Vis 2008; 14:2509-2517.


Figure 5. PDE enzymatic activities in isolated rod outer segment. Phosphodiesterase (PDE) activities were measured by thin layer chromatography using [8-3H] cGMP as the hydrolysis substrate. Rod outer segment (ROS) from dark-adapted rats were isolated, permeabilized, mixed with either 0.5 mM GTP, 0.5 mM GTPγS, or no nucleotide before they were exposed to light. Maximal PDE activity in ROS was elicited by trypsin activation (p≤0.001 for all groups).