Table 1 of Akula, Mol Vis 2008; 14:2499-2508.

Table 1. PCR primers

Target Primer sequence (5′-3′) Annealing
temp
(°C)		 Product
length
(bp)
VEGF164 F: AACCATGAACTTTCTGCTCTC 52.8 635
  R: TTGTCACATCTGCAAGTACG 52.8
NRP-1 F: GAAGGAGGGAAATAAAGCCA 52.3 572
  R: CTGATGAATCTTGTGGAGAG 50.2
Sema3A F: GACAACTTTCCTGAAAGCAC 51.9 535
  R: CCACTTTAAGGACAGTTCCA 52.1
GAPDH F: CATGTTCCAGTATGACTCTACC 52.3 829
  R: GTTGCTGTAGCCATATTCATTGTC 54.3

The table provides key details of the primers used in the RT–PCR experiments. The growth factors studied were vascular endothelial growth factor 164 (VEGF164), semaphorin IIIA (Sema3A), and neuropilin-1 (NRP-1); glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as the internal control. The length of the product of the reaction is given in base pairs (bp).

Akula, Mol Vis 2008; 14:2499-2508. http://www.molvis.org/molvis/v14/a288

©2008 Molecular Vision http://www.molvis.org/molvis/

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