Figure 5. Quantification of fluorescence intensity among GFP-labeled cells. A: Representative GFP-immunostained cells shown in A were analyzed in MetaMorph (B). The scale bar represents 10 μm for A and B. Bright cells were color coded as blue (cells 1, 2, 3 and 4), and dim cells were yellow (cells 11, 12, and 14). C: Quantification of fluorescence intensity of bright and dim cells were analyzed in four individual retinas. For each retina, 10 bright cells and 10 dim cells were chosen using the same threshold. Value represents mean±SD (n=10 for each cell subpopulation). Triple asterisks (***) indicate p<0.001. D displays the ratio of bright cell fluorescence to dim cell fluorescence intensity. For all four retinas, bright cells were 2.0±0.3 (mean±SD) fold brighter than dim cells in fluorescence intensity.