Figure 4. sCnx cells have increased ubiquitylated inclusions. Wild-type (WT; A) and calnexin (sCnx; B) cells were transfected with a His₆-myc-tagged ubiquitin expression plasmid and were stained with anti-myc antibody 24 h after transfection. Scale bar equals 10 μm. C: Cells were scored for diffuse or punctate ubiquitin stain as exhibited in (A) and (B). D: P23H rod opsin-green fluorescent protein (GFP) and myc-ubiquitin double transfected cells were scored for diffuse or punctate ubiquitin stain. Bar graphs represent an average of three independent experiments. Error bars represent ±2 SEM. Statistical analysis was performed using ANOVAR followed by posthoc tests. The asterisk indicates p<0.05.