Figure 2. Wild-type rod opsin expression in control and sCnx cells. Wild-type (WT) rod opsin-green fluorescent protein (GFP; WT-GFP) translocates to the plasma membrane of WT (A) and calnexin (sCnx) cells (B). Scale bar equals 10 μm. C: western blotting of untagged rod opsin with mAb 1D4 of 15 μg of soluble protein from WT or sCnx cell lysates (as indicated) revealed no difference in immunoreactive opsin band pattern. This representative blot was selected because of the similar rod opsin expression level between WT and sCnx cells to better demonstrate similar band pattern. The position of molecular weight markers in kDa are indicated on the left. D: Quantification of inclusion incidence for WT-opsin-GFP expressing cells after 24 h revealed no significant difference in WT-opsin-GFP aggregation in sCnx cells. Five groups of greater than 100 cells expressing WT-opsin-GFP opsin were scored for the presence of inclusions (the remaining cells had predominantly plasma membrane staining). Error bars represent ±2 Standard Error (SEM). Statistical analysis was performed by ANOVAR followed by posthoc tests. The asterisk indicates p<0.05.