Figure 1. Cnx expression in control and sCnx cells. A: Localization of calnexin (Cnx) protein in wild-type (WT) mouse embryonic fibroblasts (MEFs). B: Short Cnx localization in sCnx cells. The truncated protein shows the same staining pattern, as it still contains the N-terminal endoplasmic reticulum (ER)-targeting sequence and the C-terminal ER retention motif of full-length Cnx. Note the intensity of the sCnx staining was lower and has been adjusted to reveal the pattern. Scale bar equals 10 μm. C: Expression of ER resident chaperones Cnx and BiP in WT and sCnx cells. Western blot revealed reduced expression level and increased electrophoretic mobility of the truncated Cnx protein from sCnx cells and similar BiP levels in both cell lines.