Figure 3. Intracameral inoculation leads to an increase in CD4⁺CD25⁺FoxP3⁺ Treg. A: Flow cytometry was used to enumerate Treg in the spleen. Triple-stained splenocytes for CD4⁺, CD25⁺, and FoxP3⁺ were first gated on CD4⁺ (left) and then measured for CD25 and FoxP3 (center, right). B: ACAID induction leads to an increase in Treg in the spleen. Spleens were harvested and enumerated for Treg percentages in naïve mice or in IC inoculated mice on days 4, 8, and 12 (n=3 per time point). An asterisk indicates a statistically significant increase (p<0.5) in percentage over the naïve control. The statistical significance was calculated via Student’s t-test.