Figure 2. In vivo percentages of splenic CD4⁺ and CD8⁺ T cells and their proliferative responses in vitro during the induction and expression of ACAID. Induction of ACAID to MHC alloantigens is associated with the expansion of CD4⁺ (A) and CD8⁺ T (B) cells while expression of ACAID is associated with a reduction of T cell percentages. C57BL/6 mice were IC inoculated with allogeneic BALB/c splenocytes to examine the induction of ACAID and was followed by immunization (IMM) with BALB/c splenocytes to examine the expression of ACAID. Splenocytes were harvested at the indicated time points (n=3 per time point) for enumeration of T cell percentages via flow cytometry. Naïve spleens were similarly assessed and presented graphically on day 12. An asterisk indicates statistical significance (p<0.05) calculated via Student’s t-test. C: Induction of ACAID is associated with higher proliferation levels while expression of ACAID is associated with lower proliferation levels. T cells were harvested from hosts (n=3 per time point) at the indicated time points and stimulated in vitro to recall alloantigen (BALB/c).