Figure 1. ACAID is detectable up to 24
days following intracameral inoculation. A: ACAID induction is
typically tested approximately 14 days post intracameral inoculation.
C57BL/6 mice were intracamerally (IC) inoculated with allogeneic BALB/c
splenocytes to induce ACAID. A subsequent immunization (IMM) with
BALB/c splenocytes was administered subcutaneously on day 7 to prime IC
inoculated mice. Naïve mice were similarly primed as a positive
control. Following the challenge with BALB/c splenocytes delivered
subcutaneously to the host ear pinnae (EAR), specific ear swelling was
then measured to assess DTH. Naïve mice were also challenged as a
negative control. B: ACAID was also detected 24 days post IC
inoculation. In other mice, IMM was administered on day 12, and DTH was
subsequently tested on day 24. Each group consisted of at least five
mice or more, and an asterisk indicates statistical significance
(p<0.05) calculated via ANOVA.