Figure 5. Effect of rhBMP-2 on mRNA expression levels of MMP-2 and TIMP-2 in human sclera fibroblasts. Ethidium-bromide agarose gels indicated the level of β-actin message relative to MMP-2 (A) and TIMP-2 (B) levels from total RNA. Bar graphs revealed changes in mRNA expression (mean±standard error of the mean) where values were normalized to β-actin values and expression was stated as a ratio of optical density. The double asterisk means that the semi-quantitative RT-PCR revealed significant changes for MMP-2 and TIMP-2 with varying concentrations of rhBMP-2 (p<0.01).