Molecular Vision: Liu, Mol Vis 2008; 14:2367-2372. Table 1

Table 1. List of PCR primers for OPTN DNA sequencing in Ghanaian POAG cases and controls.

OPTN exon	Forward primer sequence	Reverse primer sequence	PCR product size (bp)	Mg²⁺ concentration
4	TAAGTATTAGCAATCGCCAA	AGTGCAAAGGGATGGCATTT	340	2.0 mM
5	CATCAGATCAAGTCCACTTT	GGAGTCTAGACACGTAAGAT	340	2.0 mM
6	ATGGTGCCCAGCCTTAGTTT	CAATCCTTGGCTTGTGTTGA	340	1.5 mM
7	CATCTGAATGTTTGGAAGCT	TATTCTGGAAAGATCCTGGT	340	1.5 mM
8	ATACTGAACAGGGCATTGTC	GTGGTTGCACAATCCTGGAA	300	1.5 mM
9	GATCCTTTATCCCAATTGTA	TTGAATTCAGTGGCTGGACT	282	2.0 mM
10	TTGATTCACCAGCCAGTCTT	GCTCACACATTAACTGGAAC	400	1.5 mM
11	TGCATTCATAAACCCTACAG	TAGGACTCCTTCAGATAAGT	400	1.5 mM
12	TTGAGAGTAAGAAATGCTAG	GATTTAGTGAAGGATTCATG	340	1.5 mM
13	ATGTTGCCCAGGCTTGTCTC	CACCATTGCTTTCCAATGCG	420	1.5 mM
14	GGATACAGCACTACCTCCTC	TCAGGAACGTCTTTGGACAG	300	1.5 mM
15	GCTCAGTGTTGTCATGTTTC	GGAATCCATTGTAGAGAATG	240	1.5 mM
16	TCGCCATCTGTTCTTCAAGT	AAAAGCACAACTCTTGGAGG	269	2.0 mM