Table 4 of Ivings, Mol Vis 2008; 14:2357-2366.

Table 4. Ratios of spliced to unspliced RNA in groups of control and splicing factor mutant cell lines for selected pre-mRNA introns

Intron and splice site Gene Control PRPF31 carrier PRPF31 severe PRPF8
U2 GT/AG ACTIN 328 406 315 402
U2 GT/AG GAPDH 1683 1385 2993 p<0.05 1433
U2 GT/AG PRPF8 198 173 184 140
U2 GT/AG RPGR 78 105 23 p<0.05 95
U2 GC/AG ZNF198 154 166 128 124 ↓
U2 GC/AG APRT 8 10 7 6.5 ↓
U12 GT/AG PLCd 18 19 21 9 ↓
U12 AT/AC SKT11 39 37 32 28 p<0.05 ↓
U12 AT/AC C3G 17 24 9 13 ↓
U12 AT/AG AP2A1 36 39 40 29 ↓

Significant differences obtained by the LSD test are highlighted in bold. A significant increase in splicing efficiency was observed for GAPDH in PRPF31 severe cell lines. In contrast a significant decrease in splicing efficiency was observed for RPGR splicing in the same cell line. In PRPF8 cell lines a significant decrease in splicing efficiency was seen for SKT11, as well as an overall decline in splicing for all U12 introns and non-canonical U2 introns.

Ivings, Mol Vis 2008; 14:2357-2366. http://www.molvis.org/molvis/v14/a272

©2008 Molecular Vision http://www.molvis.org/molvis/

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