Figure 1. Splicing ratios of U2. Splicing ratios of U2 containing introns RPGR (A) and GAPDH (B) RNA in cell lines derived from control and splicing-factor mutated cell lines. Group 1 represents controls, group 2 represents PRPF31 carriers, group 3 represents PRPF31 severe, and group 4 represents PRPF8 cell lines. The splicing ratios were obtained as described in the Methods section. Means from four to six repeat experiments are shown ±SEM. For individual cell line characteristics see Table 1.