Table 1 of Suri, Mol Vis 2008; 14:2349-2356.
Name | Sequence | Product size |
---|---|---|
Multiplex outer PCR primers | ||
CYP1B1-G61E-out F | 5′-CGAGCGAACGAGAGGTGAG-3′ | 141 bp |
CYP1B1-G61E-out R | 5′-GCTGGCCACTGTGCATGTG-3′ | |
CYP1B1-R368H & R390H-out F | 5′-GTGCAGGCAGAATTGGATCAG-3′ | 155 bp |
CYP1B1-R368H & R390H-out R | 5′-GTGTTGGCAGTGGTGGCATG-3′ | |
CYP1B1-R469W-out F | 5′-GATCCAGCTCGATTCTTGGAC-3′ | 148 bp |
CYP1B1-R469W-out R | 5′-GGTGAGCCAGGATGGAGATG-3′ | |
Multiplex inner PCR primers* | ||
CYP1B1-G61E-In F | 5′-CACTGCTTCGCAGGCTGACGTTACTGGCCCACCGCCGCCGCGT-3′ | 73 bp |
CYP1B1-G61E-In R | 5′-CGGGCCCGTTTGCGTGGCC-3′ | |
CYP1B1-R368H-In F | 5′-CACTGCTTCGCAGGCTGACGTTACTATCAGGTCGTGGGGAGGGAC-3′ | 76 bp |
CYP1B1-R368H-In R | 5′-GGTTGGGCTGGTCACCCATAC-3′ | |
CYP1B1-R390H-In F | 5′-CACTGCTTCGCAGGCTGACGTTACTCTGGCCTTCCTTTATGAAGCCA-3′ | 83 bp |
CYP1B1-R390H-In R | 5′-GAGGAATAGTGACAGGCACAAAG-3′ | |
CYP1B1-R469W-In F | 5′-CACTGCTTCGCAGGCTGACGTTACTAGTGATGATTTTTTCAGTGGGCAA-3′ | 86 bp |
CYP1B1-R469W-In R | 5′-CTGCATCTTAGAAAGTTCTTCGC-3′ | |
Biotinylatad inner primer* | 5′- CACTGCTTCGCAGGCTGACGTTACT-3′ | |
PrASE primers¶ | ||
CYP1B1-G61E-T22-G | 5′-TTACCTATGATTGATCGTGGTGATATCCGTTTGCGTGGCCACTGATCGG-3′ | |
CYP1B1-G61E-T23-A | 5′-GCTGTGGCATTGCAGCAGATTAAGGTTTGCGTGGCCACTGATCGA-3′ | |
CYP1B1-R368H-T24-G | 5′-TGACGTCATTGTAGGCGGAGAGCTAGTCACCCATACAAGGCAGAC-3′ | |
CYP1B1-R368H-T25-A | 5′-TCAATAATCAACGTAAGGCGTTCCTGTCACCCATACAAGGCAGAT-3′ | |
CYP1B1-R390H-T26-G | 5′-TTATCGGCTACATCGGTACTGACTCAGGCACAAAGCTGGAGAAGC-3′ | |
CYP1B1-R390H-T27-A | 5′-CCATTATCGCCTGGTTCATTCGTGAAGGCACAAAGCTGGAGAAGT-3′ | |
CYP1B1-R469W-T28-C | 5′-GGCGTACCTTCGCGGCAGATATAATAGTTCTTCGCCAATGCACCG-3′ | |
CYP1B1-R469W-T29-T | 5′-AACTGAGCCGTAGCCACTGTCTGTCCAGTTCTTCGCCAATGCACCA-3′ |