Figure 2. (-)-epigallocatechin gallate reduces the generation of ROS and prevents the loss of Δψm and the release of mitochondrial cytochrome c into the cytosol in HLEB-3 cells

A: The cells were pretreated with 50 μM EGCG for 1 h followed by treatment with 100 μM H₂O₂ for 2 h. The production of ROS was examined by measuring the level of ROS production using DCF-DA by flow cytometry. B: The cells were incubated with either 100 μM H₂O₂ for 6 h or pretreated 50 μM EGCG for 1 h in the presence of H₂O₂. The Δψm of cells was measured by staining with JC-1 by flow cytometry. C: After treatment for 24 h with 100 μM H₂O₂ or with 25 and 50 μM EGCG in the presence of H₂O₂, the cells were analyzed by western blot analysis. The result is one representative example of three separate experiments.