Figure 1. (-)-epigallocatechin gallate protects against H₂O₂-induced cell death in HLEB-3 cells

A: After the HLEB-3 cells were incubated with different concentrations of H₂O₂ (50, 100, and 200 μM) for 24 h or with EGCG treatment (10, 25, 50, 75, 100, and 150 μM) 1 h before H₂O₂ treatment, the viability was measured by MTT assay. B: The cell viability pretreated with EGCG at 50 μM for 1 h before exposure to 100 μM H₂O₂ was estimated using MTT assay with a time-course. The data are represented as mean±SD from three independent experiments. The asterisk indicates that p<0.05 compared to the untreated control and the sharp (hash mark) means that p<0.05 compared to the H₂O₂-treated group. C: The cells were incubated with 50 μM EGCG for 1 h then treated with 100 μM H₂O₂ for 24 h. The result is one representative example of three separate experiments.