Figure 5 of Mao, Mol Vis 2008; 14:2309-2320.


Figure 5. Reprogrammed cells responded to NDMA+glycine. A: Montage of fluorescence micrographs is shown to illustrate responses by two reprogrammed cells (arrows) in an retinal pigment epithelium (RPE) cell culture infected with RCAS-ash1. The image in the first panel was captured with longer exposure time for a clear view of the cells. Time (in seconds) after the application of the neurotransmitter is shown at the bottom of each panel. B, C: The ΔF/Fo of each of the two cells identified in A was plotted against time (in seconds) to produce a response curve. D: The ΔF/Fo a retinal cell in an E16 chick retinal cell culture was plotted against time to produce a response curve as a reference for the reprogrammed cells. E: The ΔF/Fo of a cell in the control RPE culture infected with RCAS was plotted against time to produce a response curve as a negative control. While no response was observed with RPE cells, reprogrammed cells responded to NDMA+glycine by transiently increasing Ca2+ concentrations, similar to the responses by retinal cells. The arrow in B-E points to the time at which the neurotransmitter was applied. Scale bars represents 50 μm.