Figure 4. Reprogrammed cultures lacked vimentin⁺ cells that were Müller glia-like. A, B: Immunostaining for vimentin (B) showed no cells that displayed spindle-like processes (i.e., Müller glia-like) in the control culture infected with Replication Competent Avian Splice (RCAS)-green fluorescent protein (GFP), and A is a bright-field view of the culture. C, D: Reprogrammed cultures (infected with RCAS-ash1) also lacked vimentin⁺ cells with spindle-like processes (D); C is a bright-field view of the culture. Arrows point to cells with compact cell bodies discernible under Hoffman modulation optics. E-G: A lack of vimentin⁺ cells that displayed spindle-like processes in reprogrammed culture was not due to a lack of reprogramming, because double-labeling showed that no vimentin⁺ cells spindle-like processes were detected (F) at places where a large number of calretinin⁺ cells were present (G); E is a bright-field view of the culture. Arrows point to calretinin⁺ cells. H and I are higher magnifications of F and G, respectively. Scale bars represents 50 μm.