Figure 2. Neural markers were detected in RPE cell cultures infected with RCAS-ash1 (or Replication Competent Avian Splice (RCAS)-ash1ΔC_rb) with immunocytochemistry. A: Immunostaining for calretinin showed no positive cells in a control culture infected by RCAS-green fluorescent protein (GFP). B: A large of number of calretinin+ cells were present in a culture infected by RCAS-ash1. C is a higher magnification view of a section in B. Arrows point to clusters of calretinin⁺ cells. D: MAP2⁺ cells were present in a culture infected by RCAS-ash1ΔC_rb. E: MAP2⁺ cells in RCAS-ash1ΔC_rb-infected culture displayed neuron-like morphologies. F-H: Double-labeling for calretinin (F) and visinin (G) showed that a small number of calretinin⁺ cells were also visinin⁺. H is a merge of F and G. Arrowheads in F, G, and H each point to a visinin⁺ cell that lacked calretinin immunostaining. Scale bars represents 50 μm.