Figure 12. Expression and subcellular
localization of ZO-3 in culture. RPE was isolated from E7 or E14
embryos and cultured with SF2 in the basal medium chamber and either
SF3 or rcSF3 in the apical medium chamber. The cultures were labeled
for ZO-3 (A) or double labeled for ZO-3 and ZO-1 (B).
Under standard optics (A), ZO-3 was observed along the lateral
membranes in each culture. There was no apparent effect by rcSF3 on the
distribution of ZO-3. Confocal microscopy (B) revealed the
distribution of ZO-3 and ZO-1 in the XZ plane. ZO-3 colocalized with
ZO-1 in a junctional complex at the apical end of the lateral
membranes. In (B), arrowheads that point up indicate basal
membrane, while arrowheads that point down mark apical membrane. The
scale bar represents 10 μm.