Table 1 of Johnson, Mol Vis 2008; 14:2211-2226. Table 1 of Johnson, Mol Vis 2008; 14:2211-2226.
Table 1. Troubleshooting guide for subretinal injection.
| Problem | Probable cause | Solutions |
|---|---|---|
| Lens cloudy | Lens capsule nicked during surgery | Avoid the lens. |
| No blebs | Not penetrating the retina | Press a little harder. |
| No blebs | Torn retina or retina hole; fluid leaks out quickly into vitreous | Penetrate retina in a single motion. |
| No blebs or a poorly inflated bleb | Fluid leaks out quickly into vitreous | Pause for 5–10 s before removing the blunt needle from the subretinal bleb. This allows pressure equilibration. |
| Blood | Penetrating into the choroid | Press gently. |
| Blood | Nicking the ciliary body | Sweep closer to lens. |
| Cloudy cornea | Eye was not kept moist before surgery | Apply lubricating eye drops between proparicaine and tropicamide application. |
| Air bubbles | Air lines or solutions not degassed | Degas solutions. Flush the lines and prime them with water before filling with delivery solution. |