Figure 8. Expression and death assessment in the retina following subretinal injection and electroporation of pVAX-tdTomato. A: Cross-sections of the retina were cut with a cryostat. Red fluorescence was detected in a single cell found in the RPE layer. This image is the red channel from a confocal image. B: TUNEL staining (green channel) was done in the same section. This panel shows three TUNEL-positive nuclei in a field of roughly 400 nuclei in the PhR cell layer in the image. C: A and B were merged and show the DAPI channel (blue) of the same section. The scale bar represents 50 μm.