Figure 7. Electroporation of the mouse eye viewed in cross section. A, B, and C: Demonstrated are the results of representative experiments with optimized electroporation conditions after the subretinal injection of pVAX-tdTomato [52]. The cross-sections were cut with a cryostat and nuclei stained with DAPI. A, B, and C all illustrate specific expression of tdTomato in the RPE cell layer. The red RPE cells each appear to subtend a region of about 50 nuclei in the outer nuclear layer, consistent with the known relationship of each RPE cell supporting numerous PhR cells. The scale bar represents 50 μm.