Figure 2. The subretinal injection technique. A: Position of the 34 gauge beveled needle is shown nearly tangential just before lancing the cornea. B: This schematic illustrates the position of the 35 gauge blunt needle after puncturing the neural retina and partially inflating the interphotoreceptor space (the subretinal space) to produce subretinal blebs. C: Presented is a still image from a video illustrating penetration of the cornea. D: This panel shows the positioning of the 35 gauge blunt needle in the center of the anterior chamber. E: The 35 gauge needle penetrates through the retina into the subretinal space. F: The 35 gauge needle is removed from the vitreous after subretinal injection of quantum dots. A small number of quantum dots are evident in the vitreous that generate a reddish-orange color. G: Illustrated is a fundus before subretinal injection. The retinal vessels can be readily detected in the fundus image. A ruddy red background color can be observed before injection. H: Shown is the fundus immediately after subretinal injection. The positions of three blebs surrounding the optic nerve head are located at clock face positions 4, 8, and 11. Each bleb appears puffy and gray in color with red vessels between the blebs. The optic nerve head is nearly centered in the image of the fundus. The imaged mouse eyes are about 3 mm in diameter.