Figure 1. A map of the expression plasmid, pVAX-tdTomato. This expression plasmid was created [52] by inserting tdTomato cDNA into the pVAX™200-DEST plasmid (Invitrogen). The CMV Immediate Early promoter drives expression of tdTomato. Located on the 3′ flanking side of the tdTomato cDNA is the bovine growth hormone poly(A) signal. The plasmid bears the Kanamycin resistance gene, the pUC origin of replication, and attB sites.