Figure 5. Rabbit corneal epithelium-ACMP, keratocyte-ACMP, and endothelium-ACMP scaffold was built in vitro. The cultivated rabbit corneal epithelial cells, keratocytes, and endothelial cells were positive for keratin 3, vimentin, and aquaporin A following immunofluorescence staining (A, B, and C, respectively) and these three types of cultivated cells were seeded onto ACMP. The epithelium-ACMP, keratocyte-ACMP, and endothelium-ACMP scaffold were built, respectively (D, E, and F). SEM showed that these three types of cultivated cells adhered to the surface of ACMP, and maintained their conformation (G, H, and I).