Figure 4. Retinal and optic nerve findings at various intervals after rAION induction. A: Control optic nerve from Thy1-CFP transgenic mouse; no axonal loss is detected. B: Histologicial section of the optic nerve at 21 days after rAION induction; arrows point to moderate axonal loss (maximal, 25%). C: Flat-mounted retina from control (untreated) eye of same mouse showing normal density of RGC nuclei. D. Flat-mounted retina from study eye showing RGC loss, as compared to control (C). E: Histological section of control (untreated) optic nerve from CNPase-GFP transgenic mouse. Note the complete myelinization and number of oligodendrocyte nuclei. F: Twenty-one days after rAION induction, maximal (20%–30%) oligodendrocyte loss is demonstrated.