Figure 2. RGC loss in flat-mounted retinas of Thy1-CFP mice at various intervals after crush injury. A: Control retina; no damage is detected. B: Four days after crush injury; approximately 20%–30% RGC cell loss was detected. C: Seven days after crush injury; 50% RGC loss was detected. D: Fourteen days after crush injury; maximal 75% RGC loss can be detected. Note the diffuse loss of the labeled cells.